Journal: Small (Weinheim an der Bergstrasse, Germany)

Article Title: Probiomimetics-Novel Lactobacillus-Mimicking Microparticles Show Anti-Inflammatory and Barrier-Protecting Effects in Gastrointestinal Models.

doi: 10.1002/smll.202003158

Figure Lengend Snippet: Figure 6. Cytokine production in inflamed macrophage-like dTHP-1 cells as well as in enterocyte-like Caco-2 cells. Cells were stimulated with lipopoly- saccharides (10 µg mL-1) and cotreated with probiomimetics or MVs. Supernatants were harvested after 6 or 24 h, and the protein content was analyzed using ELISA. To exclude the effects of different MV concentrations during the assembly of the probiomimetics, all results were normalized to the protein content. a) ELISA measurement of proinflammatory TNF-α released in dTHP-1 cells after 6 h. b) Release of anti-inflammatory IL-10 after 6 h. c) ELISA measurement of proinflammatory TNF-α released in dTHP-1 cells after 24 h. d) Release of anti-inflammatory IL-10 after 24 h. e) Release of proinflam- matory IL-8 in enterocyte-like Caco-2 cells after 24 h. Values represent the mean of 3–9 biological replicates with standard deviations.

Article Snippet: The supernatants were thawed and the concentrations of IL-10 and TNF-α were analyzed using Human IL-10 ELISA Set (Diaclone, Besançon, France), Human TNF-α ELISA Set (Diaclone, Besançon, France), and Human IL-8 ELISA Set (Diaclone, Besançon, France), respectively, according to the supplier’s protocols.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Cell reports. Medicine

Article Title: Targeting neoadjuvant chemotherapy-induced metabolic reprogramming in pancreatic cancer promotes anti-tumor immunity and chemo-response.

doi: 10.1016/j.xcrm.2023.101234

Figure Lengend Snippet: Figure 6. Targeting CD36 synergistically promoted AG-mediated killing of PDAC in preclinical models (A) Visual presentation of subcutaneous xenograft murine PDAC tumor models (C57 mice) for each group. (B) Measurement of tumor volumes showed CD36 blockage synergistically promoted AG-mediated killing of PDAC in subcutaneous xenograft murine PDAC tumor models. (C) Measurement of tumor weights showed CD36 blockage synergistically promoted AG-mediated killing of PDAC in subcutaneous xenograft murine PDAC tumor models (n = 5). (D) Representative IHC staining showed Ki67 expression in subcutaneous xenografts treated with different regimens. (E) t-Distributed stochastic neighbor embedding (TSNE) analyses showed the clustering for CD36+ CD8+ T cells and GZMB+ CD8+ T cells. (F) Flow cytometry revealed that more CD8+ T cells infiltrated PDAC with NAC, while the percentage of CD36+ CD8+ T cells also increased (n = 5) (mean with standard deviation). (G) ELISA results showed the combination of AG and CD36 blockade significantly improved IFN-g and tumor necrosis factor a (TNF-a) levels intratumorally (n = 5). (H) Representative image of orthotopic murine models of PDAC. (I) Kaplan-Meier curve revealed the combination of CD36 blockade and AG significantly prolonged the survival interval of mice that received orthotopic PDAC cell transplantation (n = 10). Circle or square referred to a happened event (death or censored). Censored event means the mice is still alive at the time point that we ended follow-up. (J) CD36 blockade synergistically with AG regimens optimally narrowed the PDAC tumor size in a humanized PDX model (n = 10). (K) Representative IHC staining image of CD36-high and -low PDAC. (L) Kaplan-Meier curve showed increased CD36 expression predicted worse prognosis of PDAC patients with adjuvant AG chemotherapy. The statistical sig- nificance shown in this figure was detected using t test.

Article Snippet: The ELISA kits used in the present study were as follows: Human IFN-gamma ELISA Kit (absin, abs510007); Human IL-2 ELISA Kit (Boster, EK0397); Mouse TNF alpha ELISA Kit (absin, abs520010) and Mouse IFN- gamma ELISA Kit (absin, abs520007).

Techniques: Immunohistochemistry, Expressing, Flow Cytometry, Standard Deviation, Enzyme-linked Immunosorbent Assay, Transplantation Assay, Adjuvant

Journal: EMBO Reports

Article Title: Glycogenesis and glyconeogenesis from glutamine, lactate and glycerol support human macrophage functions

doi: 10.1038/s44319-024-00278-4

Figure Lengend Snippet: Reagents and tools table

Article Snippet: IL-1β (human) ELISA set , Diaclone , 851 610 020.

Techniques: Sequencing, Control, Magnetic Beads, Recombinant, Colorimetric Assay, Staining, Reverse Transcription, Enzyme-linked Immunosorbent Assay, Software, Microscopy, Cytometry, Mass Spectrometry, Imaging, Spectrophotometry

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Relationship between circulating syndecan-1 levels (CD138s) and serum free light chains in monoclonal gammopathies

doi: 10.1186/s13046-015-0155-4

Figure Lengend Snippet: Scatter graph and correlations (R) of serum CD138 concentrations versus Serum Free Light Chain Kappa or Lambda concentrations in Intact Immunoglobulin Multiple Myeloma and Light Chain Myeloma Patients. R values were calculated by Pearson’s correlation.

Article Snippet: Serum CD138 levels were determined using ELISA kits specific for human CD138 (Diaclone Research, France).

Techniques:

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Relationship between circulating syndecan-1 levels (CD138s) and serum free light chains in monoclonal gammopathies

doi: 10.1186/s13046-015-0155-4

Figure Lengend Snippet: ROC curves depicting percentage Specificity and Sensitivity of serum CD138 concentration values in Intact Immunoglobulin Multiple Myeloma versus serum CD138 concentration values in controls, as well as serum CD138 concentration values in Light Chain Multiple Myeloma versus serum CD138 concentration values in controls.

Article Snippet: Serum CD138 levels were determined using ELISA kits specific for human CD138 (Diaclone Research, France).

Techniques: Concentration Assay

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Relationship between circulating syndecan-1 levels (CD138s) and serum free light chains in monoclonal gammopathies

doi: 10.1186/s13046-015-0155-4

Figure Lengend Snippet: Distributions of CD138 concentrations a.) Box and Whisker plot depicting the distribution of CD138 serum concentrations in Intact Immunoglobulin Multiple Myeloma Patients and Light Chain Multiple Myeloma patients. Data in the graph depict the following values: *Intact Immunoglobulin Multiple Myeloma (Median = 30.5). **Light Chain Multiple Myeloma (Mean = 24.5). b.) Scatter plot depicting CD138 concentrations among the two groups of patients compared to healthy controls. (Intact Immunoglobulin Multiple Myeloma vs Light Chain Multiple Myeloma P = 0.012; Intact Immunoglobulin Multiple Myeloma vs Controls P < 0.0001; Light Chain Multiple Myeloma vs Controls P = 0.006). Data in the graph depict the following values: *Intact Immunoglobulin Multiple Myeloma (Mean = 90.73, SEM = 15.63, SD = 103.80). **Light Chain Multiple Myeloma (Mean = 40.21, SEM = 9.47, SD = 55.22). ***Controls (Mean = 15, SEM = 1.43, SD = 9.02). (P values were calculated by Student’s Unpaired t-test).

Article Snippet: Serum CD138 levels were determined using ELISA kits specific for human CD138 (Diaclone Research, France).

Techniques: Whisker Assay

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Relationship between circulating syndecan-1 levels (CD138s) and serum free light chains in monoclonal gammopathies

doi: 10.1186/s13046-015-0155-4

Figure Lengend Snippet: Data from ROC curve analysis vs CD138 concentrations in Intact Immunoglobulin Multiple Myeloma or Light Chain Multiple Myeloma. CD138 concentrations corresponding to 95% discrimination specificity of the test are reported within each graph. The black line indicates the CD138 value corresponding to 95% specificity.

Article Snippet: Serum CD138 levels were determined using ELISA kits specific for human CD138 (Diaclone Research, France).

Techniques: